Laboratory of Functional and Molecular Membrane Physiology - FMMP

Address
Strubergasse 21, A-5020 Salzburg

Head
Univ.-Prof. Dr. Mark Ritter

Staff
Dr. Martin Jakab
Eva Iglseder, biomedical analyst (BMA)
Mag Biol Sabine Schmidt
Rer.nat MA. Christian Langelüddecke
Dr. Sofya Bulatova
Ivano Costa, Bakk. Biol

Main research areas and techniques:

• Mechanisms and functional significance of cell volume regulation
• Molecular identification and functional characterization of the non-gastric H +/K +-ATPase
• The importance of ion transport in tumor formation and apoptosis
• Regulation of cell migration and chemotaxis through ion transport
• Molecular, biophysical and pharmacological characterization of the swellung-activated anion channel and its functional significance for cell proliferation, apoptosis, chemotaxis and hormone secretion
• Electrophysiology: Patch Clamp (whole cell ionic current measurements, single ion channel analysis, membrane potential measurements, action potentials)
• Fluorescence resonance energy transfer (FRET): molecular protein-protein-interactions and protein-membrane-interactions of CFP-YFP labeled proteins and / or membranes in living cells
• Microfluorimetric techniques: intracellular measurements of ion concentrations (calcium, chloride, pH, etc.), Quenching techniques (Fura 2-manganese, mEq-Halid, calcein, FRET-based methods, etc.)
• Cell volume: measurement of cell volume and cell volume regulation with the Coulter method and simultaneous fluorescence measurement using Cell Lab Quanta zytometer (Beckman-Coulter), video imaging, deconvolution, 3D/4D rendering flow
• ELISA
• Cell migration measurements: time lapse video monitoring, chamber and gel assays
• Molecular biology: tandard methods of DNA and RNA preparation, PCR, RT-PCR, Real-Time PCR, RACE-technology, DNA cloning, promoter-luciferase assays, site-directed mutagenesis, gel electrophoresis, protein purification, Western blotting, cell transfection
• Cell culture

Laboratory for Translational Research in Immunotoxicity TIF

Address
Billrothstraße 11, A-5020 Salzburg

Head
Univ. Doz. Mag. Dr. Arnulf Josef Hartl

Staff
Dr. Tatjana Stepanoska
Martin Gaisberg
Mag Renata Sanovic
Mag Heidi Mair
Ulrike Pichler, Bakk. Biol
Kathrin Watzinger, Bakk. Biol


Main research areas and techniques:

• DNA-based vaccines for protective and therapeutic applications against allergic diseases
• Biological and immunological characterization of hypo-allergenic proteins
• In silico generated hypoallergens for specific immunotherapy (SIT)
• Investigation of environmental influences on the course of atopic diseases
• Investigation of waterfall microclimate (aerosol) on clinical, functional and molecular allergy and asthma, and stress parameters
• Protein engineering and protein purification
• Molecular biology, large-scale plasmid production, real-time PCR
• Cloning and expression
• 4-color FACS, intracellular cytokine colouration
• Cytometric Bead Arrays
• Mediator release assays (FAST, CAST, RBL)
• Lymphocyte proliferation assay
• Plethysmography
• Anaphylaxis assays (direct and passive PCA)
• Production of monoclonal antibodies
• Cell culture
• Clinical studies:
  
  Clinical study on “Splash Camp Krimml”
  www.splash-ionosol.com or www.splash-camp-krimml.at
  
  Controlled clinical study: "Stress Reduction and Short-Term Leave" in the National Park Hohe Tauern, Carinthia
  www.aquahealth.at
  
  Controlled clinical study: on "Gender, Health and Short-term Leave" in Krimml.
  (www.splashionosol.com)
  
  Preclinical study: o "Asthma Athletes" at the Oetztaler Stuibenfall

Research Institute Gastein