Forschung & Innovation
Publikationen
Intraocular tumor-associated lymphangiogenesis a novel prognostic factor for ciliary body melanomas with extraocular extension?
PMU Autor*in
L. M. Heindl
Alle Autor*innen
L. M. Heindl, T. N. Hofmann, W. Adler, H. L. Knorr, L. M. Holbach, G. O. Naumann, F. E. Kruse, C. Cursiefen
Fachzeitschrift
OPHTHALMOLOGY
Kurzfassung
PURPOSE: To evaluate whether intraocular tumor-associated lymphangiogenesis contributes to prognosis of ciliary body melanomas with extraocular extension and to study its association with other tumor characteristics. DESIGN: Nonrandomized, retrospective case series. PARTICIPANTS: Twenty consecutive patients enucleated for a malignant melanoma of the ciliary body with extraocular extension. METHODS: Lymphatic vessels were identified using lymphatic vascular endothelial-specific hyaluronic acid receptor-1 (LYVE-1) and podoplanin as specific immunohistochemical markers for lymphatic vascular endothelium. Baseline tumor characteristics included intra- and extraocular tumor size, 2009 tumor, node, metastasis (TNM) classification, route of extraocular spread, tumor cell type, mitotic rate, Ki-67 proliferation-index, microvascular patterns and density, tumor-infiltrating lymphocytes and macrophages, and expression of human leukocyte antigen (HLA) class I and insulin-like growth factor-1 receptor. Kaplan-Meier and Cox regression analyses of melanoma-specific survival were performed. MAIN OUTCOME MEASURES: Prevalence of intraocular LYVE-1(+)/podoplanin(+) lymphatic vessels and association with intraocular tumor characteristics and metastasis-free survival. RESULTS: Intraocular LYVE-1(+) and podoplanin(+) lymphatic vessels could be detected in 12 (60%) of 20 ciliary body melanomas with extraocular extension. Presence of intraocular LYVE-1(+)/podoplanin(+) lymphatic vessels was significantly associated with larger intra- (P = 0.002) and extraocular tumor size (P
Keywords
Aged Biomarkers, Tumor/metabolism Ciliary Body/metabolism/*pathology Female Histocompatibility Antigens Class I/metabolism Humans Immunoenzyme Techniques Ki-67 Antigen/metabolism *Lymphangiogenesis Lymphatic Metastasis Lymphatic Vessels/metabolism/*pathology Lymphocytes, Tumor-Infiltrating/pathology Male Melanoma/metabolism/mortality/*secondary Membrane Glycoproteins/metabolism Middle Aged Prognosis Receptor, IGF Type 1/metabolism Retrospective Studies Survival Rate Uveal Neoplasms/metabolism/mortality/*pathology Vesicular Transport Proteins/metabolism Podoplanin